Epididymal fat pads of mice
Webadipocytes per unit wet weight of epididymal fat pad and the plasma volume have been studied in lean and obese mice, and in obese mice on chronic restriction of their food intake. -- 2. There were fewer, larger adipoeytes per unit ... sions each from the entire fat pads from lean (45 mice) and ob/ob mice (9) were used for the data on adipocyte ... WebFig. 1 Standard procedure for isolating primary adipose cells from epididymal fat pads induces TNF-α transcription and protein secretion. Pools of epididymal fat pads isolated from 8–9-week-old C57BL/6J …
Epididymal fat pads of mice
Did you know?
WebMay 5, 2024 · Epididymal VAT removal surgery. Mice were anesthetized by using isoflurane. After the abdominal cavity was opened, epididymal visceral fat pads were removed and weighted (1.13 ± 0.13 g). Specifically, we carefully avoided damage to blood vessels and other organs, such as intestine and testis, as previously described . Sham … WebApr 29, 2003 · Seven epididymal fat pads were isolated from age-matched SPARC-null and wild-type mice and treated with 500 units/ml bacterial collagenase type II (Sigma), in saline with 2% BSA, with agitation for 1 h at 37°C. The filtrate was centrifuged at 800 rpm for 10 min at 4°C in a Beckman GPKR centrifuge.
WebApr 8, 2024 · In this study, we aimed to investigate the influence of casein hydrolysates (CH) containing VPP and IPP on a high fat diet (HFD)-induced obese mice and cytokine TNF-α-induced adipocytes. ... epididymal, brown scapular, subcutaneous, perirenal and mesentery fat pads were dissected and weighted, all organ and tissue samples were collected and ... WebAfter blood collection, seven different tissues of from each mouse: hippocampus, hypothalamus, heart, aorta, liver, skeletal muscle (gastrocnemius muscle), and …
WebExpressions of Several Pro-Inflammatory Cytokines Were Increased in Epididymal Fat Tissues of Lep ob/ob Mice, ... Briefly, subcutaneous fat pads isolated from the abdominal walls of eight-week-old male WT/ND mice were transplanted into the subcutaneous area below the skin on the back of six-week-old Lep ob/ob mice. The fat pads isolated ... Webparametrial fat pads of cold acclimated female CD1 mice and female C57BL/6 mice also contain small areas of brown tissue, but these mice have less brown tissue than female BALB/c mice. However, no brown tissue can be observed in the epididymal fat pad of male BALB/c mice even after cold acclimation.
WebApr 29, 1997 · For light microscopy and immunohistochemical analysis, fat pads derived from implanted preadipocytes and epididymal fat pads were paraffin-embedded after 24 h of fixation in buffered formalin. Four …
WebNov 28, 2003 · Standard isolation of primary adipose cells from mouse epididymal fat pads induces inflammatory mediators and down-regulates adipocyte genes J Biol Chem. 2003 Nov 28;278(48):47585-93. doi: 10.1074/jbc.M305257200. ... Mice Mice, Inbred C57BL Monosaccharide Transport Proteins / metabolism ... john satcher attorneyWebPathologic alterations have been found in the epididymal fat pad of five strains of spontaneously obese or diabetic mice and in one strain of normal mice made obese by a single injection of goldthioglucose. - Histologic abnormalities were observed in the epididymal fat pad of all obese and diabetic mice. how to get to bhutan from manilaWebApr 13, 2024 · a, b Body weight (a, n = 10) and epididymal fat pad (b, n = 10) of C57BL/6 mice orally gavaged with H 2 O alone or supplemented with cysteine (4 mmol/kg per day) for 60 days. how to get to bhutan from philippinesWebApr 13, 2024 · a, b Body weight (a, n = 10) and epididymal fat pad (b, n = 10) of C57BL/6 mice orally gavaged with H 2 O alone or supplemented with cysteine (4 mmol/kg per day) for 60 days. how to get to bhutan from singaporeWebThe tissues were obtained from the epididymal fat pads of fat, lean and normal strains of mice and from sixty-one human subjects undergoing various abdominal operations. In both the mice and the humans, the adipose tissue from obese individuals contained slightly more fat and less protein and water than that from lean individuals. how to get to bhutan from sydneyWebIn vivo, serum samples and omental adipose tissue from humans as well as epididymal fat pads from mice were collected to detect miR-27a expression. In vitro, 3T3-L1 preadipocytes and mature adipocytes were treated with TNF-α to induce apoptosis and transfected with a mimic for overexpressing miR-27a-3p. The results showed that miR-27a was ... how to get to bibury from londonWebJianPi-QingHua formula attenuates nonalcoholic fatty liver disease by regulating the AMPK/SIRT1/NF-κB pathway in high-fat-diet-fed C57BL/6 mice Pharm Biol. 2024 Dec;61 ... Compared with the HFD group, JPQH could reduce body weight, epididymal fat mass, blood glucose and liver weight (p < 0.05), and markedly decreased the levels of serum … how to get to bicester village by train