WebThis approach requires application of mitochondrial inhibitors-oligomycin to block ATP synthase, FCCP-to make the inner mitochondrial membrane permeable for protons and allow maximum electron flux through the electron transport chain, and rotenone and antimycin A-to inhibit complexes I and III, respectively. WebB. The experiment was a ‘failure’ in that FCCP, oligomycin and DisruptMiNo all have identical effects upon both ΔΨm and the ability of mitochondria to synthesise ATP; C. DisruptMiNo was considered to have been proven ‘successful’ and achieved a positive outcome by significantly reducing the E max of the FCCP only Log DR relationship;
FCCP Optimization using the Cell Mito Stress Test …
WebAug 2, 2024 · Extracellular flux analysis and xCT expression confirmed that FCCP/oligomycin induced mitochondrial dysfunction in all cell lines. Across the four cell lines, mitochondrial dysfunction resulted... WebMar 7, 2016 · Oligomycin (oligo*; item number 11341) was also obtained from Cayman Chemical Company (Ann Arbor, MI, USA), as was citreoviridin (citre; item number 11319). CCCP, citreoviridin, DNP, FCCP, oligomycin and oligomycin A stock solutions were prepared by dissolving the respective chemicals in DMSO. the butlers hotel leeds
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WebApr 7, 2024 · ATP-linked Respiration was determined by the difference in OCR after the addition of oligomycin. Proton Leakage was determined by subtracting OCR with rotenone/antimycin A from the OCR after the addition of oligomycin. Respiratory capacity was determined by subtracting OCR with rotenone/antimycin A from OCR after the … WebApr 30, 2014 · The oligomycin-sensitivity conferring protein (OSCP) of the mitochondrial FOF1 ATP synthase has long been recognized to be essential for the coupling of proton transport to ATP synthesis. Located on top of the catalytic F1 sector, it makes stable contacts with both F1 and the peripheral stalk, ensuring the structural and functional … WebNov 16, 2014 · Oligomycin A (1uM), FCCP (200nM for PC3-Epi and PC3-EMT, and 400nM for PrECs), rotenone (2uM), antimycin A (2uM), and 2-DG (100mM) were used to evaluate mitochondrial respiratory capacity and glycolytic capacity. The data were normalized by the amount of protein present in each well. We confirmed that normalization to the number of … the butler service gmbh hechingen