NettetFor cutting white adipose tissue, embedding in OCT is fine. I snap freeze the WAT immediately in -80°C isopentane. Then take a small piece from the tissue and embedd it in OCT for cryocutting ... Nettet8. apr. 2024 · Thus, HFD caused macrophage infiltration in white adipose tissue such as epididymal adipose in obese mice, whereas such inflammatory infiltration was …
Decreased adipogenesis and adipose tissue in mice with …
NettetAdipose tissue is a complex tissue composed of adipocytes and the stromal vascular fraction which includes a heterogeneous population of preadipocytes, blood cells, endothelial cells, and macrophages. In the present chapter, methods are detailed to generate purified mature adipocytes from white adipose tissue by using enzymatic … Nettet20. mar. 2024 · Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBPβ and in turn by promoting the expression of its downstream effectors, such as PPARγ and C/EBPα. snl beyonce single ladies
Adipose tissue at single-cell resolution - ScienceDirect
NettetThe same is true in mice and rats (Gesta et al., 2006). Regional differences in gene expression, replicative potential, and capacity for adipogenesis persist despite maintenance under identical culture conditions for many weeks in colonies derived from single preadipocytes (Djian et al., 1983, 1985; Kirkland et al., 1990; Tchkonia et al., 2002). Nettet16. mar. 2024 · We additionally provide a framework for mouse–human comparison in studies of adipose tissue that will be an important resource for groups hoping to translate mouse findings to human treatments. Nettet30. nov. 2024 · Moreover, adipose tissue expression of genes in this pathway correlated with body weight extremes in a cohort of genetically diverse mice and with BMI in 2 independent cohorts of humans. These data implicate a signaling axis that may explain reduced hormone-stimulated lipolysis in obesity and resistance to therapeutic … snl birthday gifts